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Time‐sharing evolution and sensitivity enhancements in 2D HSQC‐TOCSY and HSQMBC experiments
Author(s) -
Nolis Pau,
Pérez Miriam,
Parella Teodor
Publication year - 2006
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1898
Subject(s) - heteronuclear single quantum coherence spectroscopy , chemistry , chemical shift , sensitivity (control systems) , proton , nitrogen , measure (data warehouse) , coupling constant , range (aeronautics) , biological system , analytical chemistry (journal) , two dimensional nuclear magnetic resonance spectroscopy , stereochemistry , data mining , nuclear physics , chromatography , physics , computer science , organic chemistry , materials science , particle physics , electronic engineering , biology , engineering , composite material
Modifications of time‐shared (TS) HSQC‐like experiments originally developed by Griesinger and co‐workers (Sattler M, Maurer M, Schleucher J, Griesinger C. J. Biomol. NMR 1995; 5: 97) are proposed to extract different types of information from a single NMR pulse scheme. It is shown that simultaneous acquisition of 1 H, 13 C and 1 H, 15 N HSQC‐TOCSY and HSQMBC experiments can afford experimental sensitivity enhancements of 20–40% with respect to the separate acquisition of individual 13 C or 15 N data. In addition, the incorporation of a number of independent editing elements can be easily used for different purposes, for instance, to assign unambiguously 1 H, 13 C, and 15 N chemical shifts, to differentiate directly from relayed cross‐peaks, or to measure simultaneously long‐range proton–carbon and proton–nitrogen coupling constants. The suggested methodologies can be applied to many different classes of nitrogen‐containing compounds and illustrative examples are provided for the peptide cyclosporine as a demonstration of the performance of such experiments. Copyright © 2006 John Wiley & Sons, Ltd.