z-logo
Premium
Paramagnetic tagging of diamagnetic proteins for solution NMR
Author(s) -
RodriguezCastañeda Fernando,
Haberz Peter,
Leonov Andrei,
Griesinger Christian
Publication year - 2006
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1811
Subject(s) - chemistry , diamagnetism , paramagnetism , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , computational chemistry , stereochemistry , magnetic field , condensed matter physics , physics , quantum mechanics
In this article, approaches towards the paramagnetic tagging of diamagnetic proteins are reviewed. Alignment can be achieved by adding paramagnetic fusion proteins or peptides to the C ‐ or the N ‐terminus or by attaching paramagnetic tags to Cystein residues. Applications for the study of homodimer structures and protein/ligand interactions, as well as protein domain dynamics, are reviewed. Copyright © 2006 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here
Accelerating Research

Address

John Eccles House
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom