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Paramagnetic tagging of diamagnetic proteins for solution NMR
Author(s) -
RodriguezCastañeda Fernando,
Haberz Peter,
Leonov Andrei,
Griesinger Christian
Publication year - 2006
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1811
Subject(s) - chemistry , diamagnetism , paramagnetism , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , computational chemistry , stereochemistry , magnetic field , condensed matter physics , physics , quantum mechanics
In this article, approaches towards the paramagnetic tagging of diamagnetic proteins are reviewed. Alignment can be achieved by adding paramagnetic fusion proteins or peptides to the C ‐ or the N ‐terminus or by attaching paramagnetic tags to Cystein residues. Applications for the study of homodimer structures and protein/ligand interactions, as well as protein domain dynamics, are reviewed. Copyright © 2006 John Wiley & Sons, Ltd.