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Solid‐state NMR investigation of sodium nucleotide complexes
Author(s) -
Grant Christopher V.,
McElheny Dan,
Frydman Veronica,
Frydman Lucio
Publication year - 2006
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1750
Subject(s) - chemistry , sodium , nucleotide , nuclear magnetic resonance spectroscopy , solid state nuclear magnetic resonance , uridine , crystallography , context (archaeology) , magic angle spinning , stereochemistry , inorganic chemistry , rna , organic chemistry , nuclear magnetic resonance , biochemistry , paleontology , physics , biology , gene
Solid‐state NMR has been used to analyze the chemical environments of sodium sites in powdered crystalline samples of sodium nucleotide complexes. Three of the studied complexes have been previously characterized structurally by crystallography (disodium deoxycytidine‐5′‐monophosphate heptahydrate, disodium deoxyuridine‐5′‐monophosphate pentahydrate and disodium adensoine‐5′‐triphosphate trihydrate). For these salts, the nuclear quadrupole coupling parameters measured by 23 Na multiple‐quantum magic‐angle‐spinning NMR could be readily correlated with sodium ion coordination environments. Furthermore, two complexes that had not been previously characterized structurally, disodium uridine‐3′‐monophosphate and a disodium uridine‐3′‐monophosphate/disodium uridine‐2′‐monophosphate mix, were identified by solid‐state NMR. A spectroscopic assignment of the four sites of an additional salt, disodium adensoine‐5′‐triphosphate trihydrate, is also presented and discussed within the context of creating a general approach for the spectroscopic assignment of multiple sites in sodium nucleotide complexes. Copyright © 2006 John Wiley & Sons, Ltd.