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Incorporation of spin‐labelled amino acids into proteins
Author(s) -
Becker Christian F. W.,
Lausecker Kester,
Balog Mária,
Kálai Tamás,
Hideg Kálmán,
Steinhoff HeinzJürgen,
Engelhard Martin
Publication year - 2005
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1688
Subject(s) - chemistry , site directed spin labeling , nitroxide mediated radical polymerization , spin label , amino acid , electron paramagnetic resonance , membrane protein , biochemistry , recombinant dna , combinatorial chemistry , biophysics , membrane , organic chemistry , nuclear magnetic resonance , polymerization , physics , radical polymerization , polymer , biology , gene
The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor‐made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site‐specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site‐specifically spin‐labelled proteins. Copyright © 2005 John Wiley & Sons, Ltd.