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High‐field spin‐label EPR of lipid membranes
Author(s) -
Marsh Derek,
Kurad Dieter,
Livshits Vsevolod A.
Publication year - 2005
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1680
Subject(s) - chemistry , electron paramagnetic resonance , membrane , spin label , field (mathematics) , spin (aerodynamics) , nuclear magnetic resonance , condensed matter physics , chemical physics , biochemistry , thermodynamics , physics , mathematics , pure mathematics
High‐field EPR of spin‐labelled lipid chains has proved to be an extremely productive means for biophysical investigations of phospholipid bilayer membranes. Results on the following three topics are reviewed: 1. Non‐axial ordering of lipid chains in cholesterol‐containing membranes; 2. Transmembrane polarity profiles and water penetration in lipid membranes; 3. Role of HF‐EPR in multi‐frequency spectral simulations to investigate lipid‐chain dynamics in membranes. These results are obtained from phosphatidylcholine spin probes with the nitroxide systematically stepped down the sn ‐2 chain, in fully hydrated bilayer membranes of dimyristoyl phosphatidylcholine (DMPC)–containing cholesterol. Copyright © 2005 John Wiley & Sons, Ltd.