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NMR spectroscopic characterization of the membrane affinity of polyols
Author(s) -
Fischer Daniela,
Geyer Armin
Publication year - 2005
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1653
Subject(s) - chemistry , phosphatidylcholine , membrane , characterization (materials science) , polarity (international relations) , ligand (biochemistry) , molecule , dipole , nuclear magnetic resonance spectroscopy , residual , phospholipid , stereochemistry , crystallography , receptor , organic chemistry , nanotechnology , biochemistry , materials science , cell , algorithm , computer science
Residual dipolar couplings (RDCs) are applied here for the analysis of weak, transient binding events between phosphatidylcholine bilayers and polyols. Large signal responses are observed even for low percentages of ‘ligand‐receptor complexes,’ making RDCs a sensitive tool for the analysis of molecular recognition events. The different degree of alignment in solution can be compared as a result of the calculation of the alignment tensor elements. By varying polarity and/or charge of the molecules under investigation, nonspecific hydrophobic effects can be excluded. Copyright © 2005 John Wiley & Sons, Ltd.