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Metabolic profile of lettuce leaves by high‐field NMR spectra
Author(s) -
Sobolev Anatoli P.,
Brosio Elvino,
Gianferri Raffaella,
Segre Anna L.
Publication year - 2005
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1618
Subject(s) - chemistry , heteronuclear single quantum coherence spectroscopy , carotenoid , proton nmr , aqueous solution , carbon 13 nmr , nmr spectra database , amino acid , spectral line , two dimensional nuclear magnetic resonance spectroscopy , chromatography , organic chemistry , stereochemistry , biochemistry , physics , astronomy
A detailed analysis of the proton high‐field NMR spectra of aqueous and organic extracts of lettuce leaves is reported for the first time. A combination of COSY, TOCSY, 1 H– 13 C HSQC, 1 H– 13 C HMBC bidimensional sequences and DOSY was used to assign each spin system and to separate the components of the complex patterns. A large number of water‐soluble metabolites belonging to different classes such as carbohydrates, polyols, organic acids and amino acids were fully assigned. Moreover, the complex spectra of metabolites extracted in organic solvents belonging to sterols, fatty acids, diacylglycerophospholipids, galactosyldiacylglycerols, sulpholipids, pheophytins, carotenoids and hydrocarbons were also assigned. Copyright © 2005 John Wiley & Sons, Ltd.