Measurement of 1 J (N i ,C α i ), 1 J (N i ,C′ i −1 ), 2 J (N i ,C α i −1 ), 2 J (H N i ,C′ i −1 ) and 2 J (H N i ,C α i ) values in 13 C/ 15 N‐labeled proteins

Use of partial or selective 13 C/ 15 N labeling of specific amino acid residues in a given protein to measure the values of 1 J ( 15 N i , 13 C α i ), 2 J ( 1 H N , 13 C α i ), 2 J ( 15 N i , 13 C α i −1 ), 1 J ( 15 N i , 13 C′ i −1 ) and 2 J ( 1 H N , 13 C′ i −1 ) is described. This was achieved by recording a sensitivity‐enhanced 2D [ 15 N– 1 H] HSQC experiment, without mixing the spin states of C α and C′ during the course of entire experiment. Copyright © 2005 John Wiley & Sons, Ltd.