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Application of NMR screening techniques for observing ligand binding with a protein receptor
Author(s) -
Shimotakahara Sakurako,
Furihata Kazuo,
Tashiro Mitsuru
Publication year - 2005
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1492
Subject(s) - chemistry , ligand (biochemistry) , nuclear magnetic resonance spectroscopy , pulsed field gradient , pulse sequence , ribonuclease , nuclear magnetic resonance , proton nmr , small molecule , receptor , molecule , analytical chemistry (journal) , stereochemistry , chromatography , biochemistry , rna , organic chemistry , physics , gene
Water ligand observed via gradient spectroscopy (WaterLOGSY), saturation transfer difference and NOE pumping NMR techniques were used to identify ligand binding with a receptor. Although these experiments were originally designed to observe ligands in complexes, their application is limited by the affinity of ligands towards target molecules. Here the improved WaterLOGSY pulse sequence was developed by incorporating the double pulsed field gradient spin‐echo and gradient‐tailored excitation WATERGATE sequences. The efficiency of these ligand‐observed NMR screening techniques was investigated using the ribonuclease T 1 ‐inhibitor system. Copyright © 2004 John Wiley & Sons, Ltd.