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Heating caused by radiofrequency irradiation and sample rotation in 13 C magic angle spinning NMR studies of lipid membranes
Author(s) -
Dvinskikh Sergey V.,
Castro Vasco,
Sandström Dick
Publication year - 2004
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1477
Subject(s) - chemistry , decoupling (probability) , spinning , magic angle spinning , irradiation , analytical chemistry (journal) , spectral line , rotor (electric) , heteronuclear molecule , magic angle , membrane , proton nmr , rotation (mathematics) , carbon 13 nmr , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , chromatography , organic chemistry , physics , polymer chemistry , biochemistry , geometry , mathematics , quantum mechanics , control engineering , astronomy , nuclear physics , engineering
Application of rapid sample rotation and radiofrequency irradiation in magic angle spinning (MAS) NMR of lipid bilayers can significantly increase the sample temperature. In this work, we studied the extent of heating during the acquisition of 1 H‐decoupled 13 C MAS spectra of hydrated dimyristoylphosphatidylcholine (DMPC) in the L α phase. First, we describe a simple procedure for determining the increase in temperature by observing the shift of the 1 H water signal. The method is then used to identify and assess the various factors that contribute to the sample heating. The important factors discussed in this paper include: (i) the spinning speed, (ii) the variable‐temperature gas pressure, (iii) the rotor geometry, (iv) the power, duration and frequency of the radiofrequency irradiation and (v) the hydration level. A comparison of different heteronuclear decoupling schemes in terms of their ability to produce highly resolved 13 C spectra of DMPC is also reported. Copyright © 2004 John Wiley & Sons, Ltd.