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Improvement of double pulsed field gradient spin‐echo ROESY experiments for identification of bound waters
Author(s) -
Furihata Kazuo,
Shimotakahara Sakurako,
Tashiro Mitsuru
Publication year - 2003
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1272
Subject(s) - chemistry , pulse sequence , coherence (philosophical gambling strategy) , sequence (biology) , pulsed field gradient , excitation , sensitivity (control systems) , spin echo , heteronuclear single quantum coherence spectroscopy , field (mathematics) , analytical chemistry (journal) , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , biological system , molecule , stereochemistry , chromatography , physics , organic chemistry , biology , medicine , biochemistry , quantum mechanics , electronic engineering , magnetic resonance imaging , engineering , radiology , mathematics , pure mathematics
A novel pulse sequence incorporating the double pulsed field gradient spin‐echo (DPFGSE) and the gradient‐tailored excitation WATERGATE techniques is presented that has particular use for identifying bound waters in 15 N‐labeled macromolecules. This sequence, DPFGSE–ROESY–HSQC, affords greater spectral sensitivity than the DPFGSE–ROESY–HMQC experiment which was previously presented and is consequently useful for rapidly obtaining reliable information for characterizing macromolecular bound water molecules. A significant enhancement in the sensitivity is achieved by using the gradient‐tailored excitation WATERGATE sequence in the reverse INEPT step as it allows the use of much higher receiver gains. Since coherence selection is not used, the sequence has improved sensitivity together with less spectral artifacts. The advantage of this pulse sequence is illustrated using 15 N‐labeled ribonuclease T 1 . Copyright © 2003 John Wiley & Sons, Ltd.