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Investigation of intracellular phosphate pools of the green alga Chlorella using 31 P nuclear magnetic resonance
Author(s) -
Sianoudis J.,
Mayer A.,
Leibfritz D.
Publication year - 1984
Publication title -
organic magnetic resonance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0030-4921
DOI - 10.1002/mrc.1270220603
Subject(s) - intracellular , extracellular , chlorella , phosphate , sugar phosphates , intracellular ph , polyphosphate , nuclear magnetic resonance spectroscopy , chemistry , biochemistry , algae , biophysics , biology , botany , stereochemistry
31 P NMR measurements were performed in order to study the intracellular phosphate pools of the green alga Chlorella fusca . Cells of asynchronous cultures contain a large pool of polyphosphates, whereas the amount of polyphosphates in cells of synchronous cultures depends on the state of differentiation. The assignment of extracellular and intracellular inorganic orthophosphate is achieved by changing the pH, whereas the intracellular compartmentation of inorganic orthophosphates and sugar phosphates is shown during irradiation of the cells with light within the NMR spectrometer.