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15 N NMR spectroscopy 31 —preliminary study on the insulin‐a chain (bunte salt form)
Author(s) -
Hull William E.,
Büllesbach Erika,
Wieneke HansJoachim,
Zahn Helmut,
Kricheldorf Hans R.
Publication year - 1981
Publication title -
organic magnetic resonance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0030-4921
DOI - 10.1002/mrc.1270170204
Subject(s) - chemistry , salt (chemistry) , amide , solvent , nuclear magnetic resonance spectroscopy , derivative (finance) , resolution (logic) , insulin , chromatography , spectroscopy , organic chemistry , medicine , physics , quantum mechanics , artificial intelligence , computer science , financial economics , economics
The Bunte salt derivative was prepared from bovine insulin‐A isolated from natural sources, and was purified by chromatography. Natural abundance 40.55 MHz 15 N NMR spectra were measured in water and dimethyl sulphoxide. The latter solvent gave the best resolution, and 20 of the 22 possible amide nitrogen signals were detected. Several model oligopeptides were also measured and their usefulness for assignments is discussed.