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Examination of the aspirin acetylation site of human serum albumin by 13 C NMR spectroscopy
Author(s) -
Gerig J. T.,
Katz K. E.,
Reinheimer J. D.,
Sullivan Glenn R.,
Roberts John D.
Publication year - 1981
Publication title -
organic magnetic resonance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0030-4921
DOI - 10.1002/mrc.1270150210
Subject(s) - tetramethylsilane , chemistry , acetamide , human serum albumin , acetylation , resonance (particle physics) , nuclear magnetic resonance spectroscopy , aspirin , nuclear magnetic resonance , spectroscopy , methyl group , stereochemistry , organic chemistry , chromatography , group (periodic table) , biochemistry , physics , particle physics , quantum mechanics , gene
Human serum albumin has been specifically acetylated using aspirin in which the methyl carbon of the acetyl group was enriched to 90% 13 C. A single resonance at 23.13 ppm downfield from tetramethylsilane was observed in 13 C differences spectra obtained at both 25.2 and 45.3 MHz. Chemical shift studies of several model compounds suggest that this is the resonance position to be expected for an acetamide group exposed to solvent. The line width observed for the enriched methyl resonance is consistent with free rotation of the methyl group.