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Coupling of HPLC and NMR 1—analysis of flowing liquid chromatographic fractions by proton magnetic resonance
Author(s) -
Buddrus Joachim,
Herzog Helmut
Publication year - 1980
Publication title -
organic magnetic resonance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0030-4921
DOI - 10.1002/mrc.1270130220
Subject(s) - high performance liquid chromatography , pulse sequence , chemistry , chromatography , analytical chemistry (journal) , free induction decay , laser linewidth , nmr spectra database , pulse (music) , spectral line , proton nmr , nuclear magnetic resonance , fourier transform , nuclear magnetic resonance spectroscopy , detector , spin echo , magnetic resonance imaging , physics , laser , organic chemistry , optics , medicine , astronomy , quantum mechanics , radiology
A procedure is described which allows the recording of 1 H NMR spectra from high performance liquid chromatography (HPLC) fractions flowing through the magnetic field with a velocity of either 0.05 or 0.25 cm/s. The NMR spectra are obtained by the pulse Fourier transform technique; the beginning and the termination of the pulse sequence is timed according either to the chromatogram from a UV detector or to the free induction decay of the appropriate HPLC fraction. The linewidth of the signals in the NMR spectra is between 1.5 and 2.0 Hz.