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15 N nuclear magnetic resonance studies of [1‐ 15 N]nicotinamide adenine dinucleotides
Author(s) -
Oppenheimer Norman J.,
Davidson Robert M.
Publication year - 1980
Publication title -
organic magnetic resonance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0030-4921
DOI - 10.1002/mrc.1270130104
Subject(s) - chemistry , nad+ kinase , pyridine , moiety , nicotinamide , nicotinamide adenine dinucleotide , ring (chemistry) , stereochemistry , redox , nuclear magnetic resonance , medicinal chemistry , inorganic chemistry , biochemistry , enzyme , organic chemistry , physics
The synthesis of [1‐ 15 N]nicotinamide adenine dinucleotide is described. Chemical shift data from 15 N NMR studies are presented for the pyridine ring nitrogen of labeled NAD and related compounds. The results indicate a 15 N label in the N‐1 position to be highly sensitive to the redox‐state of the pyridine moiety, with an upfield shift of over 100 ppm observed upon reduction of NAD + to NADH. The feasibility of conducting 15 N NMR studies of pyridine nucleotide binding to dehydrogenases is discussed.