Nitrogen‐15 n.m.r. studies of  13 C,  15 N labeled arginine | Zendy

London R. E. | Zendy; Walker T. E. | Zendy; Whaley T. W. | Zendy; Matwiyoff N. A. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Nitrogen‐15 n.m.r. studies of 13 C, 15 N labeled arginine

Author(s) -

London R. E.,

Walker T. E.,

Whaley T. W.,

Matwiyoff N. A.

Publication year - 1977

Publication title -

organic magnetic resonance

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.483

H-Index - 72

eISSN - 1097-458X

pISSN - 0030-4921

DOI - 10.1002/mrc.1270091011

Subject(s) - protonation , chemistry , deprotonation , proton , tautomer , spectral line , nitrogen , resonance (particle physics) , urea , arginine , crystallography , stereochemistry , medicinal chemistry , analytical chemistry (journal) , amino acid , organic chemistry , ion , physics , biochemistry , quantum mechanics , astronomy , particle physics

15 N n.m.r. spectra of [ 13 C‐2, 3‐ 15 N 2 ‐ guanidino ]arginine and [ 13 C, 15 N 2 ] urea were obtained in D 2 O and H 2 O at a variety of pH values both with and without proton decoupling. The effects of the proton exchange rate are readily observable in the proton coupled 15 N spectra. When the guanidino group is deprotonated (p K = 12.5), the terminal nitrogens give a single resonance 6.6 ppm downfield of the protonated species, indicating a rapid tautomeric exchange. The observed NH and CN couplings are compared with calculated values, and good agreement is found for 1 J (CN) using a Blizzard–Santry type calculation. The ramifications of the proton exchange on 15 N n.m.r. spectra of amino acids and peptides are discussed.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research