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A carbon‐13 nuclear magnetic resonance study of the cobalt (II)–Nicotinamide adenine dinucleotide complexes
Author(s) -
Torreilles Jean,
De Paulet André Crastes,
Platzer Nicole
Publication year - 1977
Publication title -
organic magnetic resonance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0030-4921
DOI - 10.1002/mrc.1270091007
Subject(s) - nicotinamide adenine dinucleotide , nicotinamide adenine dinucleotide phosphate , cofactor , chemistry , nad+ kinase , cobalt , ion , flavin adenine dinucleotide , nucleotide , crystallography , stereochemistry , inorganic chemistry , enzyme , biochemistry , oxidase test , organic chemistry , gene
The addition of Co 2+ to D 2 O solutions of Nicotinamide Adenine Dinucleotide induces modifications of the 13 C n.m.r. signal line widths of these coenzymes. Analysis of the observed paramagnetic effects shows that both states (reduced and oxidized) and both conformations (folded or unfolded) of these coenzymes always allow two types of interactions between the cobalt ion and the nucleotide, namely where Co 2+ is linked to the phosphate groups and to nitrogen‐7 of the adenine nucleus. Moreover, the metal ion also interacts with the adenine NH 2 in the NAD + folded conformation. These natural abundance 13 C n.m.r. experiments allowed a fairly precise description of the Nicotinamide Adenine Dinucleotide—Co 2+ interactions.