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The use of DSS as an internal standard in PMR studies of nucleic acid interactions
Author(s) -
Live David. H.,
Chan Sunney I.
Publication year - 1973
Publication title -
organic magnetic resonance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0030-4921
DOI - 10.1002/mrc.1270050606
Subject(s) - chemistry , sulfonic acid , aqueous solution , silylation , sodium , sodium salt , nucleic acid , ammonium chloride , purine , inorganic chemistry , organic chemistry , biochemistry , catalysis , enzyme
Two commonly used PMR internal reference compounds, 3‐(trimethyl silyl) propane sulfonic acid sodium salt (DSS) and 3‐(trimethyl silyl) sodium propionate (TSP), have been shown to interact with purine in aqueous solution, causing significant shifts in the resonance position of their reference protons. Tetramethyl ammonium chloride (TMACl) appears to be a more suitable reference compound for such studies.