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Carbon‐13 NMR study of the B9(Asp) mutant of human insulin: Sequence‐specific carbon‐13 assignments and structural information
Author(s) -
Kristensen Søren M.,
Led Jens J.
Publication year - 1995
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260330610
Subject(s) - chemistry , mutant , human insulin , dimer , carbon 13 nmr , sequence (biology) , chemical shift , stereochemistry , crystallography , insulin , biochemistry , gene , organic chemistry , medicine , endocrinology
The resonances of the proton‐bearing 13 C nuclei at natural abundance were assigned for the solution dimer of the B9(Asp) mutant of human insulin. The assignments are based on a combination of direct and relayed 1 H 13 C correlated spectra, i.e. SQC, SQC‐HOHAHA and HMQC‐COSY, and the previously assigned 1 H spectrum. The study proves valuable not only because of the 13 C assignments, but also because it provides a verification, a completion and a few corrections of the previous 1 H assignments. Finally, the obtained 13 C assignments support the previously reported correlation between the secondary 13 C α and 13 C β chemical shifts and the secondary structure of proteins.