Premium
NMR visibility of 35 Cl − in human erythrocytes
Author(s) -
Riddell Frank G.,
Zhou Zhe
Publication year - 1995
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260330111
Subject(s) - chemistry , intracellular , ion , chloride , extracellular , nmr spectra database , spectral line , laser linewidth , analytical chemistry (journal) , crystallography , biochemistry , chromatography , physics , organic chemistry , astronomy , optics , laser
Abstract Contrary to previous reports, Cl − is visible by 35 Cl NMR inside human erythrocytes. In spectra run at 49.01 MHz the intracellular chloride has a linewidth at half‐height of ca 700 Hz and shows double Lorentzian character. Mn 2+ was used to relax the extracellular 35 Cl − to allow the intracellular 35 Cl − to be seen. The experimental protocol described should allow NMR observation of anion transport rates in human erythrocytes.