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1 H 2D‐NMR characterization of Ni(II)‐substituted azurin from Pseudomonas aeruginosa
Author(s) -
Moratal JoséMaría,
Salgado Jesús,
Donaire Antonio,
Jiménez Hermas R.,
Castells Josep,
Martínezferrer Maríajosé
Publication year - 1993
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260311310
Subject(s) - chemistry , azurin , two dimensional nuclear magnetic resonance spectroscopy , spectral line , nmr spectra database , crystallography , proton nmr , metal , carbon 13 nmr satellite , stereochemistry , hyperfine structure , nuclear magnetic resonance spectroscopy , fluorine 19 nmr , copper , organic chemistry , physics , atomic physics , astronomy
1 H two‐dimensional NMR experiments on nickel(II)‐substituted azurin have been successfully applied. Despite the short relaxation times of the hyperfine‐shifted resonances, the combined use of NOESY and COSY spectra allowed the assignment of 15 resonances belonging to the metal‐coordinated residues Gly‐45, His‐46, His‐117 and Met‐121. Even in the case of the two broad and furthest downfield resonances, the NOESY spectra were successful in assigning these signals to the β‐CH 2 protons of Cys‐112. The protons of the non‐coordinated residues Met‐13, Phe‐15 and Trp‐48 were also assigned via NOESY, COSY and TOCSY experiments.