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Copper‐cobalt superoxide dismutase: A re‐examination of the 1 H NMR spectrum through a novel selectively deuteriated derivative
Author(s) -
Bertini Ivano,
Piccioli Mario,
Scozzafava Andrea,
Viezzoli Maria Silvia
Publication year - 1993
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260311306
Subject(s) - chemistry , cobalt , deuterium , superoxide dismutase , copper , proton , derivative (finance) , proton nmr , two dimensional nuclear magnetic resonance spectroscopy , stereochemistry , inorganic chemistry , enzyme , organic chemistry , atomic physics , physics , quantum mechanics , financial economics , economics
Through 1D and 2D NOESY experiments, samples of copper‐cobalt superoxide dismutase in which the histidines had been completely deuteriated except for the β‐CH 2 protons were investigated. In this way a simplified spectrum was obtained which allowed the detection of the His NHs of the cobalt domain and their assignment through the kinetics of the proton‐deuterium exchange. Further, the β‐CH 2 protons of Asp 83 were stereospecifically assigned through T 1 measurements. Some backbone protons were also assigned.