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1 H 3D NOE‐NOE spectrum of met‐cyanomyoglobin: The first 3D NMR spectrum of a paramagnetic protein
Author(s) -
Banci Lucia,
Bermel Wolfgang,
Luchinat Claudio,
Pierattelli Roberta,
Tarchi Dario
Publication year - 1993
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260311304
Subject(s) - chemistry , hyperfine structure , paramagnetism , diamagnetism , spectrum (functional analysis) , envelope (radar) , nuclear magnetic resonance , spectral line , molecular physics , atomic physics , physics , magnetic field , condensed matter physics , telecommunications , radar , quantum mechanics , computer science , astronomy
A 3D NOE‐NOE spectrum of the paramagnetic protein met‐cyanomyoglobin has been successfully obtained. The protein shows hyperfine shifted signals. Planes at their frequencies show clean cross peaks with signals of nearby protons. Connectivities can be detected in‐plane at frequencies corresponding to hyperfine broadened signals beneath the diamagnetic envelope, whose connectivities may be difficult to observe otherwise. The limits of observability of cross peaks are discussed by means of spectral simulations.