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High‐resolution solid‐state 13 C CP/MAS NMR study of scleroglucan hydration
Author(s) -
Bardet M.,
Rousseau A.,
Vincendon M.
Publication year - 1993
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260311003
Subject(s) - chemistry , anhydrous , solid state nuclear magnetic resonance , random coil , aqueous solution , nuclear magnetic resonance spectroscopy , molecule , crystallography , solid state , nuclear magnetic resonance , stereochemistry , organic chemistry , circular dichroism , physics
Scleroglucan is a branched poiysaccharide, soluble in water. Solid‐state and solution 13 C NMR were used to follow the fixation of water molecules on anhydrous scleroglucan and the evolution of the scleroglucan—water complex from the solid state to the gel and solution state. Determination of both chemical shift values and relaxation times ( T CH D , T 1pH and T 1C ) for anhydrous and hydrated scleroglucans permit the observation of a transition between the two forms. The fixation of water leads to an increase in the solid‐state order, in the form of a triple‐helix association, and at the same time an increase in the interchain distance and in the molecular motion. The gel state obtained for a water‐scleroglucan composition of 90:10 is characterized by an important macromolecular rigidity which implies the use of the solid‐state NMR techniques. Solution 13 C NMR can be utilized to characterize the primary structure and the triple‐helix random‐coil conformational transition.