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13 C NMR spectroscopy of keratan sulphates: Assignments for the reduced form of a repeat unit tetrasaccharide derived from keratan sulphate by keratanase digestion and partial assignments for two fucosylated pentasaccharides
Author(s) -
Huckerby Thomas N.,
Dickenson John M.,
Tai GuiHua,
Lauder Robert M.,
Brown Gavin M.,
Nieduszynski Ian A.
Publication year - 1993
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260310416
Subject(s) - tetrasaccharide , chemistry , keratan sulfate , nuclear magnetic resonance spectroscopy , spectroscopy , stereochemistry , analytical chemistry (journal) , glycosaminoglycan , chromatography , organic chemistry , biochemistry , polysaccharide , physics , quantum mechanics , chondroitin sulfate
A full assignment of the 60°C 13 C NMR spectrum for the reduced form of a repeat unit β‐linked tetrasaccharide derived by keratanase digestion from keratan sulphate, GlcNAc(6SO 4 )β 1–3 Gal(6SO 4 )β 1–4 GlcNAc(6SO 4 )β 1–3 ‐Gal‐ol, was achieved via two‐dimensional C/H correlation and 1 H COSY experiments, and a complete first‐order analysis of the 600 MHz proton spectrum. Partial 13 C assignments for two fucosylated keratan sulphate pentasc‐charides derived using hydrazinolysis and nitrous acid treatment are discussed.