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Applications of gradient‐selective COSY and DQCOSY to brucine and gibberellic acid
Author(s) -
Carpenter T. A.,
Colebrook L. D.,
Hall L. D.,
Pierens G. K.
Publication year - 1992
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260300813
Subject(s) - brucine , chemistry , gibberellic acid , spectral line , analytical chemistry (journal) , resolution (logic) , chromatography , physics , botany , biochemistry , germination , astronomy , artificial intelligence , computer science , strychnine , biology
High‐quality gradient‐selective COSY spectra were recorded at 300 MHz for brucine and gibberellic acid and double‐quantum filtered gradient‐selective COSY spectra for brucine, using a single‐frequency, high‐resolution 1 H probe modified by the addition of an unshielded z‐gradient coil set. Data were efficiently recorded using buffered acquisition with a single transient per increment in 1 t, in times much lower than those required for phase‐cycled COSY, and with no loss of information. Gradient‐selective COSY90, COSY45 and long‐range COSY45 experiments, and the correlations leading to chemical shift assignments for gibberellic acid, are reported.