Premium
13 C‐selective, 1 H‐detected 2D { 1 H, 13 C} correlation spectra of oligosaccharides
Author(s) -
Poppe Leszek,
van Halbeek Herman
Publication year - 1991
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260290816
Subject(s) - chemistry , anomer , heteronuclear single quantum coherence spectroscopy , spectral line , trisaccharide , resolution (logic) , two dimensional nuclear magnetic resonance spectroscopy , pulse sequence , nmr spectra database , analytical chemistry (journal) , nuclear magnetic resonance , stereochemistry , crystallography , chromatography , physics , astronomy , artificial intelligence , computer science
Two‐dimensional 1 H‐detected { 1 H, 13 C} shift correlation spectra of oligosaccharides usually suffer from low resolution in the 13 C frequency domain. This drawback can be overcome through a simple modification of the HSQC experiment, namely by implementing a selective 13 C 180° pulse. It is illustrated for the mixture of the α‐and β‐anomers of the trisaccharide sialyl‐α(2→6)‐lactose that 13 C‐selective HSQC allows both one‐bond and multiple‐bond { 1 H, 13 C} correlation spectra to be obtained with high resolution in the 13 C domain.