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Structure of the De‐ O ‐acetylated glucuronoxylomannan from Cryptococcus neoformans serotype D, as determined by 2D NMR spectroscopy
Author(s) -
Skelton Marshall A.,
Cherniak Robert,
Poppe Leszek,
van Halbeek Herman
Publication year - 1991
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260290808
Subject(s) - chemistry , heteronuclear molecule , nuclear magnetic resonance spectroscopy , cryptococcus neoformans , spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , stereochemistry , acetylation , crystallography , microbiology and biotechnology , biochemistry , physics , biology , quantum mechanics , gene
The primary structure of the de‐ O ‐acetylated glucuronoxylomannan isolated from Cryptococcus neoformans serotype D was characterized de novo by NMR spectroscopy. Assignment of the 1 H and 13 C spectra of the polysaccharide involved a combination of 2D NMR experiments including double‐quantum‐filtered { 1 H, 1 H} COSY, { 1 H, 1 H} TOCSY and 1 H‐detected { 1 H, 13 C} heteronuclear multiple quantum coherence spectroscopy. The sequence of the glycosyl residues, including the linkage positions, was deduced by { 1 H, 1 H} rotating‐frame NOE (ROESY) spectroscopy. The serotype‐D glucuronoxylomannan was found to be a branched polymer with a repeating pentasaccharide unit of the following structure:.