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Heteronuclear directed site‐selective NOESY spectroscopy by polarization transfer or multiple quantum filtering
Author(s) -
Volk A.,
Mispelter J.,
Dimicoli J. L.,
Papamichael E.,
Sakarellos C.
Publication year - 1988
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260260117
Subject(s) - heteronuclear molecule , chemistry , two dimensional nuclear magnetic resonance spectroscopy , pulse sequence , nuclear magnetic resonance spectroscopy , molecule , sequence (biology) , spectroscopy , nuclear overhauser effect , stereochemistry , nuclear magnetic resonance , quantum mechanics , physics , organic chemistry , biochemistry
Two methods are examined which allow a NOESY experiment to be performed involving only a specific site of the molecule labelled by a heteronucleus. They involve the merging of a classical phase‐sensitive NOESY experiment with either an INEPT sequence, which transfers 1 H magnetization to a coupled heteronucles, or a multiple quantum filtering sequence which allows the selection of only the 1 H resonances which are coupled to the heteronucles. The two methods are first compared on two small molecules, non‐labelled alanine and [U‐ 13 C]alanine, particularly with respect to the signal‐to‐noise ratio. An example of the application of these methods on a 25000 molecular weight protein system (elastase‐peptide complex) is also given. The limitations of the methods, in particular in terms of T 2 , are discussed.