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Quantitative analysis of pyrrolizidine alkaloid mixtures from Senecio vulgaris by carbon‐13 nuclear magnetic resonance spectroscopy
Author(s) -
Pieters L. A. C.,
Vlietinck A. J.
Publication year - 1987
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260250103
Subject(s) - chemistry , pyrrolizidine , pyrrolizidine alkaloid , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , alkaloid , spectroscopy , senecio , carbon 13 nmr , analytical chemistry (journal) , stereochemistry , organic chemistry , botany , physics , quantum mechanics , biology
A 13 C NMR method has been developed for obtaining simultaneously structural and quantitative information on mixtures of pyrrolizidine alkaloids from Senecio vulgaris L. (Compositae). The samples contained seneciphylline, senecionine, retrorsine and the corresponding E geometrical isomers, spartioidine, integerrimine and usaramine. The spin‐lattice relaxation times were measured and, after establishing the instrumental requirements for quantitative 13 C FT NMR, all the alkaloids of the mixture could be determined. Quantitative 1 H and 13 C FT NMR analyses were carried out, and the advantages and limitations of these methods are discussed.