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Correlated 1 H 13 C NMR spectroscopy of peracetylated glycosphingolipids at 500 MHz
Author(s) -
Dabrowski Janusz,
Dabrowski Ursula,
Hanfland Peter,
Kordowicz Maria,
Hull William E.
Publication year - 1986
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260240820
Subject(s) - chemistry , ceramide , spectroscopy , nuclear magnetic resonance spectroscopy , membrane , dept , stereochemistry , biochemistry , apoptosis , physics , quantum mechanics
The assignments of the sugar and ceramide 13 C and 1 H signals of peracetylated ceramide penta‐ and deca‐saccharide from rabbit erythrocyte membranes, viz. Galα(1→3)Galβ(→4)GlcNAcβ(1→3)Galβ(1→4)Glcβ(1→1) Cer (Cer=ceramide) and Galα(1→3)Galβ(1→4)GlcNAcα(1→3[)Galβ(1→4)GlcNAcβ(1→6)]GalNAcβ(1→4)GlcNAcβ(1→3)Galβ(1→4)Glcβ(1→1)Cer, were obtained by the combined use of homo‐ and hetero‐nuclear shift‐correlated 2D and DEPT spectra. A few ambiguities resulting from exact coincidence of 1 H resonances were resolved by relying on reference data for similar environments.