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1 H Assignments of glycopeptide antibiotics by double quantum NMR and relayed correlation spectroscopy
Author(s) -
Fesik Stephen W.,
Perun Thomas J.,
Thomas Alford M.
Publication year - 1985
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1260230809
Subject(s) - chemistry , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , spectroscopy , spectral line , carbon 13 nmr satellite , glycopeptide antibiotic , nuclear magnetic resonance , fluorine 19 nmr , stereochemistry , physics , quantum mechanics , bacteria , staphylococcus aureus , vancomycin , biology , genetics
Two‐dimensional double quantum NMR and relayed correlation spectroscopy were used to complete the 1 H assignments of a series of glycopeptide antibiotics. The double quantum NMR experiments were particularly useful in assigning the complicated aromatic region of the spectra by identifying the coupled protons with small differences in chemical shifts, and simplifying the contour maps by selectively enhancing the cross‐peaks corresponding to either the ortho ‐( J ≈8 Hz) or meta ‐( J ≈2 Hz) coupled protons. In addition, from the double quantum NMR experiments, protons belonging to the same spin system were unambiguously identified, even though not directly coupled. The connectivities between remote nuclei belonging to the same network were also established through relayed correlation spectroscopy.