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Methyl TROSY: explanation and experimental verification
Author(s) -
Ollerenshaw Jason E.,
Tugarinov Vitali,
Kay Lewis E.
Publication year - 2003
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1256
Subject(s) - chemistry , macromolecule , deuterium , relaxation (psychology) , heteronuclear single quantum coherence spectroscopy , dipole , nuclear magnetic resonance , protein dynamics , pulse sequence , chemical physics , analytical chemistry (journal) , computational chemistry , molecular dynamics , nuclear magnetic resonance spectroscopy , atomic physics , chromatography , physics , stereochemistry , organic chemistry , psychology , social psychology , biochemistry
In TROSY experiments, relaxation interference effects are exploited to produce spectra with improved resolution and signal‐to‐noise. Such experiments cannot be explained using the standard product operator formalism, but must instead be analyzed at the level of individual density matrix elements. Herein we illustrate this point using an example from our recent work on a TROSY 1 H– 13 C correlation experiment for methyl groups in large proteins. Methyl groups are useful spectroscopic probes of protein structure and dynamics because they are found throughout the critical core region of a folded protein and their resonances are intense and well dispersed. Additionally, it is relatively easy to produce highly deuterated protein samples that are 1 H, 13 C labeled at selected methyl positions, facilitating studies of high molecular weight systems. Methyl groups are relaxed by a network of 1 H– 1 H and 1 H– 13 C dipolar interactions, and in the macromolecular limit the destructive interference of these interactions leads to unusually slow relaxation for certain density matrix elements. It is this slow relaxation that forms the basis for TROSY experiments. We present a detailed analysis of evolution and relaxation during HSQC and HMQC pulse schemes for the case of a 13 C 1 H 3 spin system attached to a macromolecule. We show that the HMQC sequence is already optimal with respect to the TROSY effect, offering a significant sensitivity enhancement over HSQC at any spectrometer field strength. The gain in sensitivity is established experimentally using samples of two large proteins, malate synthase G (81.4 kDa) and ClpP protease (305 kDa), both highly deuterated and selectively 1 H, 13 C‐labeled at isoleucine δ methyl positions. Copyright © 2003 John Wiley & Sons, Ltd.