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Critical investigation of coupled liquid chromatography–NMR spectroscopy in pharmaceutical impurity identification
Author(s) -
Sharman Gary J.,
Jones Ian C.
Publication year - 2003
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1196
Subject(s) - chemistry , impurity , nuclear magnetic resonance spectroscopy , sensitivity (control systems) , instrumentation (computer programming) , chromatography , proton nmr , analytical chemistry (journal) , spectroscopy , fluorine 19 nmr , identification (biology) , carbon 13 nmr , organic chemistry , physics , botany , quantum mechanics , electronic engineering , computer science , engineering , biology , operating system
In this paper, we investigate the application of coupled LC–NMR to the identification of low‐level impurities. We consider the absolute sensitivity of the technique with our instrumentation, and how this is degraded by peak broadening on and after column, and we compare the sensitivity and other aspects of LC–NMR with a more classical approach of impurity isolation and tube NMR. We show that despite the undoubted advantages of LC–NMR in many situations, for the identification of very low‐level impurities it may not always be the most efficient overall approach when all factors are considered. Copyright © 2003 John Wiley & Sons, Ltd.