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A one‐shot sequence for high‐resolution diffusion‐ordered spectroscopy
Author(s) -
Pelta Michelle D.,
Morris Gareth A.,
Stchedroff Marc J.,
Hammond Stephen J.
Publication year - 2002
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1107
Subject(s) - chemistry , pulse sequence , diffusion , pulsed field gradient , analytical chemistry (journal) , coherence (philosophical gambling strategy) , resolution (logic) , nuclear magnetic resonance spectroscopy , sequence (biology) , spectral line , spectroscopy , field (mathematics) , nuclear magnetic resonance , computational physics , chromatography , molecule , stereochemistry , physics , biochemistry , mathematics , organic chemistry , quantum mechanics , artificial intelligence , astronomy , pure mathematics , computer science , thermodynamics
Diffusion‐ordered spectroscopy (DOSY) is a powerful method for the NMR analysis of mixtures such as crude synthetic products, biofluids or biological extracts. Mixtures can be analysed without the need for any physical separation, and the method requires only standard NMR pulsed field gradient hardware. Existing pulse sequences for DOSY require extensive and time‐consuming phase cycling for clean results. A new sequence is reported here which allows clean spectra with good lineshapes to be obtained using as little as one transient per gradient value. Asymmetric bipolar field gradient pulse pairs are used in conjunction with extra balancing gradient pulses, selecting a unique coherence transfer pathway but minimizing eddy current effects and field‐frequency lock disturbance. Using the new sequence, high‐resolution proton DOSY spectra can be obtained in less than 1 min. Copyright © 2002 John Wiley & Sons, Ltd.