Premium
1 H and 13 C NMR study of 2‐hydroxyglutaric acid and its lactone
Author(s) -
Bal Dominika,
GryffKeller Adam
Publication year - 2002
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.1053
Subject(s) - chemistry , lactone , metabolite , chemical shift , coupling constant , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , computational chemistry , nuclear magnetic resonance , organic chemistry , biochemistry , physics , particle physics
Determination of the level and absolute configuration of 2‐hydroxyglutaric acid in a patient's urine is a method of diagnosing two metabolic diseases. Such a determination can be done with the aid of NMR spectroscopic methods. In this paper the careful interpretation of 1 H and 13 C NMR spectra of this metabolite and its lactone measured under conditions used in biomedical assays is reported. The 1 H chemical shifts and spin–spin coupling constants were derived using the total lineshape analysis method. Copyright © 2002 John Wiley & Sons, Ltd.