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Cell lines of the same anatomic site and histologic type show large variability in intrinsic radiosensitivity and relative biological effectiveness to protons and carbon ions
Author(s) -
Flint David B.,
Bright Scott J.,
McFadden Conor H.,
Konishi Teruaki,
Ohsawa Daisuke,
Turner Broderick,
Lin Steven H.,
Grosshans David R.,
Chiu HuaSheng,
Sumazin Pavel,
Shaitelman Simona F.,
Sawakuchi Gabriel O.
Publication year - 2021
Publication title -
medical physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.473
H-Index - 180
eISSN - 2473-4209
pISSN - 0094-2405
DOI - 10.1002/mp.14878
Subject(s) - radiosensitivity , relative biological effectiveness , clonogenic assay , ion , dna repair , linear energy transfer , irradiation , dna damage , nuclear medicine , cell culture , radiobiology , biology , dna , chemistry , radiochemistry , genetics , physics , medicine , nuclear physics , organic chemistry
Purpose To show that intrinsic radiosensitivity varies greatly for protons and carbon (C) ions in addition to photons, and that DNA repair capacity remains important in governing this variability. Methods We measured or obtained from the literature clonogenic survival data for a number of human cancer cell lines exposed to photons, protons (9.9 keV/μm), and C‐ions (13.3–77.1 keV/μm). We characterized their intrinsic radiosensitivity by the dose for 10% or 50% survival (D 10% or D 50% ), and quantified the variability at each radiation quality by the coefficient of variation (COV) in D 10% and D 50% . We also treated cells with DNA repair inhibitors prior to irradiation to assess how DNA repair capacity affects their variability. Results We found no statistically significant differences in the COVs of D 10% or D 50% between any of the radiation qualities investigated. The same was true regardless of whether the cells were treated with DNA repair inhibitors, or whether they were stratified into histologic subsets. Even within histologic subsets, we found remarkable differences in radiosensitivity for high LET C‐ions that were often greater than the variations in RBE, with brain cancer cells varying in D 10% (D 50% ) up to 100% (131%) for 77.1 keV/μm C‐ions, and non‐small cell lung cancer and pancreatic cancer cell lines varying up to 55% (76%) and 51% (78%), respectively, for 60.5 keV/μm C‐ions. The cell lines with modulated DNA repair capacity had greater variability in intrinsic radiosensitivity across all radiation qualities. Conclusions Even for cell lines of the same histologic type, there are remarkable variations in intrinsic radiosensitivity, and these variations do not differ significantly between photon, proton or C‐ion radiation. The importance of DNA repair capacity in governing the variability in intrinsic radiosensitivity is not significantly diminished for higher LET radiation.