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Integration of a fluorescence detection system and a laminate‐based disposable microfluidic chip
Author(s) -
Irawan Rudi,
Chuan Tjin Swee,
Yaw Fu Chit
Publication year - 2005
Publication title -
microwave and optical technology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.304
H-Index - 76
eISSN - 1098-2760
pISSN - 0895-2477
DOI - 10.1002/mop.20852
Subject(s) - microfluidics , detector , materials science , fluorescence , microwave , microfluidic chip , chip , optoelectronics , substrate (aquarium) , lab on a chip , optical fiber , optics , nanotechnology , engineering , electrical engineering , telecommunications , physics , oceanography , geology
A fluorescence‐detection system for immunoassay applications in a laminate‐based microfluidic chip has been developed and tested. Blue‐LED, plastic fiber, CCD detector, mylar‐D, and fluorescein are used as an excitation source, a light coupler, a fluorescence detector, a microfluidic substrate, and a sample, respectively. The system is able to detect from 1 to 1000 μg/L fluorescein. A method to avoid cross talk is also presented. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 456–460, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20852
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