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Portulaca oleracea L . Extract Ameliorates Intestinal Inflammation by Regulating Endoplasmic Reticulum Stress and Autophagy
Author(s) -
Zhang Ziwei,
Qiao Dan,
Zhang Yali,
Chen Qian,
Chen Yujun,
Tang Yingjue,
Que Renye,
Chen Ying,
Zheng Lie,
Dai Yancheng,
Tang Zhipeng
Publication year - 2022
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202100791
Subject(s) - endoplasmic reticulum , autophagy , microbiology and biotechnology , inflammation , protein kinase r , in vivo , western blot , unfolded protein response , kinase , thapsigargin , p38 mitogen activated protein kinases , apoptosis , biology , chemistry , protein kinase a , pharmacology , immunology , biochemistry , cyclin dependent kinase 2 , gene
Scope To investigate the role of endoplasmic reticulum stress (ERS)‐induced autophagy in inflammatory bowel disease (IBD) and the intervention mechanism of Portulaca oleracea L . (POL) extract, a medicinal herb with anti‐inflammatory, antioxidant, immune‐regulating, and antitumor properties, in vitro and in vivo. Methods and Results An IL‐10‐deficient mouse model is used for in vivo experiments; a thapsigargin (Tg)‐stimulated ERS model of human colonic mucosal epithelial cells (HIECs) is used for in vitro experiments. The levels of ERS‐autophagy‐related proteins are examined by immunofluorescence and Western blot. Cellular ultrastructure is assessed with transmission electron microscopy. POL extract promotes a healing effect on colitis by regulating ERS‐autophagy through the protein kinase R‐like endoplasmic reticulum kinase (PERK)‐eukaryotic initiation factor 2α (eIF2α)/Beclin1‐microtubule‐associated protein light chain 3II (LC3II) pathway. Conclusion Overall, the results of this study further confirm the anti‐inflammatory mechanism and protective effect of POL extract and provide a new research avenue for the clinical treatment of IBD.