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Fucose as a Cleavage Product of 2’Fucosyllactose Does Not Cross the Blood‐Brain Barrier in Mice
Author(s) -
Rudloff Silvia,
Kuntz Sabine,
Borsch Christian,
Vazquez Enrique,
Buck Rachael,
Reutzel Martina,
Eckert Gunter Peter,
Kunz Clemens
Publication year - 2021
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202100045
Subject(s) - cleavage (geology) , fucose , chemistry , blood–brain barrier , microbiology and biotechnology , endocrinology , immunology , biochemistry , biology , central nervous system , glycoprotein , paleontology , fracture (geology)
Scope To further examine the role of the human milk oligosaccharide 2’fucosyllactose (2´FL) and fucose (Fuc) in cognition. Using 13 C‐labeled 2’FL,thestudy previously showed in mice that 13 C‐enrichment of the brain is not caused by 13 C 1 ‐2´FL itself, but rather by microbial metabolites. Here, the study applies 13 C 1 ‐Fuc in the same mouse model to investigate its uptake into the brain. Methods and Results Mice received 13 C 1 ‐Fuc via oral gavage (2 mmol 13 C 1 ‐Fuc/kg ‐1 body weight) or intravenously (0.4 mmol/kg ‐1 body weight). 13 C‐enrichment is measured in organs, including various brain regions, biological fluids and excrements. By EA‐IRMS, the study observes an early rise of 13 C‐enrichment in plasma, 30 min after oral dosing. However, 13 C‐enrichment in the brain does not occur until 3‐5 h post‐dosing, when the 13 C‐Fuc bolus has already reached the lower gut. Therefore, the researcher assume that 13 C‐Fuc is absorbed in the upper small intestine but cannot cross the blood‐brain barrier which is also observed after intravenous application of 13 C 1 ‐Fuc. Conclusions Late 13 C‐enrichment in the rodent brain may be derived from 13 C 1 ‐Fuc metabolites derived from bacterial fermentation. The precise role that Fuc or 2´FL metabolites might play in gut‐brain communication needs to be investigated in further studies.