Premium
Effects of Hesperidin Consumption on the Cardiovascular System in Pre‐ and Stage 1 Hypertensive Subjects: Targeted and Non‐Targeted Metabolomic Approaches (CITRUS Study)
Author(s) -
PlaPagà L.,
Pedret A.,
Valls R. M.,
CalderónPérez L.,
Llauradó E.,
Companys J.,
MartínLuján F.,
Moragas A.,
Canela N.,
Puiggròs F.,
Caimari A.,
del Bas J. M.,
Arola L.,
Solà R.,
MaynerisPerxachs J.
Publication year - 2021
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202001175
Subject(s) - hesperidin , hesperetin , urine , metabolite , metabolomics , pharmacology , blood pressure , medicine , chemistry , chromatography , pathology , alternative medicine
Scope The aim of the present work is to determine new biomarkers of the biological effects of hesperidin in orange juice (OJ) applying a non‐targeted metabolomics approach validated by targeted metabolomics analyses of compliance biomarkers. Methods and Results Plasma/serum and urine targeted (HPLC‐MS/MS) and untargeted ( 1 H‐NMR) metabolomics signatures are explored in a subsample with pre‐ and stage‐1 hypertension subjects of the CITRUS study (N = 159). Volunteers received 500 mL day −1 of control drink, OJ, or hesperidin‐enriched OJ (EOJ) for 12‐weeks. A 6‐h postprandrial study is performed at baseline. Targeted analyses reveals plasma and urine hesperetin 7‐O‐β‐ d ‐glucuronide as the only metabolite differing between OJ and EOJ groups after 12‐weeks consumption, and in urine is correlated with a decreased systolic blood pressure level. The non‐targeted approach shows that after single dose and 12‐weeks consumption of OJ and EOJ change several metabolites related with an anti‐inflammatory and antioxidant actions, lower blood pressure levels and uremic toxins. Conclusions Hesperetin 7‐O‐β‐ d ‐glucuronide can be a candidate marker for distinguishing between the consumption of different hesperidin doses at 12‐weeks consumption as well as a potential agent mediating blood pressure reduction. Moreover, changes in different endogenous metabolites can explain the mechanisms of action and the biological effects of hesperidin consumption.