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Oral Intake of Lumisterol Affects the Metabolism of Vitamin D
Author(s) -
Kotwan Julia,
Kühn Julia,
Baur Anja C.,
Stangl Gabriele I.
Publication year - 2021
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202001165
Subject(s) - calcitriol , vitamin d and neurology , cyp24a1 , chemistry , vitamin , endocrinology , medicine , calcitriol receptor , metabolism , downregulation and upregulation , biochemistry , biology , gene
Scope The treatment of food with ultraviolet‐B (UV‐B) light to increase the vitamin D content is accompanied by the formation of photoisomers, such as lumisterol 2 . The physiological impact of photoisomers is largely unknown. Methods and Results Three groups of C57Bl/6 mice are fed diets containing 50 µg kg ‐1 deuterated vitamin D 3 with 0, 50 (moderate‐dose) or 2000 µg kg ‐1 (high‐dose) lumisterol 2 for four weeks. Considerable quantities of lumisterol 2 and vitamin D 2 are found in the plasma and tissues of mice fed with 2000 µg kg ‐1 lumisterol 2 but not in those fed 0 or 50 µg kg ‐1 lumisterol 2 . Mice fed with 2000 µg kg ‐1 lumisterol 2 showed strongly reduced deuterated 25‐hydroxyvitamin D 3 (–50%) and calcitriol (–80%) levels in plasma, accompanied by downregulated mRNA abundance of c ytochrom P450 ( Cyp ) 27b1 and upregulated Cyp24a1 in the kidneys. Increased tissue levels of vitamin D 2 were also seen in mice in a second study that are kept on a diet with 0.2% UV‐B exposed yeast versus those fed 0.2% untreated yeast containing iso‐amounts of vitamin D 2 . Conclusion High doses of lumisterol 2 can enter the body, induce the formation of vitamin D 2 , reduce the levels of 25(OH)D 3 and calcitriol and strongly impact the expression of genes involved in the degradation and synthesis of bioactive vitamin D.