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Species Differences in in vitro and Estimated in vivo Kinetics for Intestinal Microbiota Mediated Metabolism of Acetyl‐deoxynivalenols
Author(s) -
Jin Jing,
Spenkelink Albertus,
Beekmann Karsten,
Baccaro Marta,
Xing Fuguo,
Rietjens Ivonne M. C. M.
Publication year - 2021
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202001085
Subject(s) - acetylation , lachnospiraceae , in vivo , in vitro , biology , metabolism , gut flora , biochemistry , feces , chemistry , microbiology and biotechnology , genetics , gene , 16s ribosomal rna , firmicutes
Scope Deoxynivalenol (DON) and its acetylated derivatives 3‐acetyl‐DON (3‐Ac‐DON) and 15‐acetyl‐DON (15‐Ac‐DON) are important mycotoxins of concern in the modern food chain. Methods and Results The present study reveals that the rate of de‐acetylation in in vitro anaerobic fecal incubations decreased in the order rat > mouse > human > pig for 3‐Ac‐DON, and mouse > human > rat > pig for 15‐Ac‐DON. The ratio between the de‐acetylation rate of 3‐Ac‐DON and 15‐Ac‐DON varies with the species. Scaling of the kinetic parameters to the in vivo situation results in catalytic efficiencies decreasing in the order human > rat > pig > mouse for 3‐Ac‐DON and human > pig > rat > mouse for 15‐Ac‐DON. The results obtained indicate that in mice, 3‐Ac‐DON can be fully deconjugated while 15‐Ac‐DON cannot. In rats, pigs, and humans, both 3‐Ac‐DON and 15‐Ac‐DON can be totally transformed by gut fecal microbiota during the estimated intestinal residence time. A correlation analysis between the deacetylation rate and the relative abundance of the microbiome suggests Lachnospiraceae may be involved in the deacetylation process. Conclusion It is concluded that interspecies differences in deacetylation of acetylated DONs exist but that in risk assessment assumption of complete intestinal deconjugation provides an adequate approach.