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Identification and Characterization of IgE‐Reactive Proteins and a New Allergen (Cic a 1.01) from Chickpea ( Cicer arietinum )
Author(s) -
Wangorsch Andrea,
Kulkarni Anuja,
Jamin Annette,
Spiric Jelena,
Bräcker Julia,
Brockmeyer Jens,
Mahler Vera,
BlancaLópez Natalia,
Ferrer Marta,
Blanca Miguel,
Torres Maria,
Gomez Paqui,
Bartra Joan,
GarcíaMoral Alba,
Goikoetxea María J.,
Vieths Stefan,
Toda Masako,
Zoccatelli Gianni,
Scheurer Stephan
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202000560
Subject(s) - vicilin , allergen , immunoglobulin e , pathogenesis related protein , storage protein , sensitization , allergy , pollen , chemistry , immunology , biology , biochemistry , botany , gene , antibody , gene expression
Scope Chickpea ( Cicer arietinum ) allergy has frequently been reported particularly in Spain and India. Nevertheless, chickpea allergens are poorly characterized. The authors aim to identify and characterize potential allergens from chickpea. Methods and Results Candidate proteins are selected by an in silico approach or immunoglobuline E (IgE)‐testing. Potential allergens are prepared as recombinant or natural proteins and characterized for structural integrity by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE), circular dichroism (CD)‐spectroscopy, and mass spectrometry (MS) analysis. IgE‐sensitization pattern of Spanish chickpea allergic and German peanut and birch pollen sensitized patients are investigated using chickpea extracts and purified proteins. Chickpea allergic patients show individual and heterogeneous IgE‐sensitization profiles with extracts from raw and boiled chickpeas. Chickpea proteins pathogenesis related protein family 10 (PR‐10), a late embryogenesis abundant protein (LEA/DC‐8), and a vicilin‐containing fraction, but not 2S albumin, shows IgE reactivity with sera from chickpea, birch pollen, and peanut sensitized patients. Remarkably, allergenic vicilin, DC‐8, and PR‐10 are detected in the extract of boiled chickpeas. Conclusion Several IgE‐reactive chickpea allergens are identified. For the first time a yet not classified DC‐8 protein is characterized as minor allergen (Cic a 1). Finally, the data suggest a potential risk for peanut allergic patients by IgE cross‐reactivity with homologous chickpea proteins.