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A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure
Author(s) -
Beckmann Manfred,
Wilson Thomas,
Zubair Hassan,
Lloyd Amanda J.,
Lyons Laura,
Phillips Helen,
Tailliart Kathleen,
Gregory Nicholas,
Thatcher Rhys,
GarciaPerez Isabel,
Frost Gary,
Mathers John M.,
Draper John
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202000517
Subject(s) - biomarker , urine , urinalysis , biomarker discovery , metabolomics , chromatography , chemistry , proteomics , biochemistry , gene
Scope Metabolites derived from individual foods found in human biofluids after consumption could provide objective measures of dietary intake. For comprehensive dietary assessment, quantification methods would need to manage the structurally diverse mixture of target metabolites present at wide concentration ranges. Methods and results A strategy for selection of candidate dietary exposure biomarkers is developed. An analytical method for 62 food biomarkers is validated by extensive analysis of chromatographic and ionization behavior characteristics using triple quadrupole mass spectrometry. Urine samples from two food intervention studies are used: a controlled, inpatient study ( n = 19) and a free‐living study where individuals ( n = 15) are provided with food as a series of menu plans. As proof‐of‐principle, it is demonstrated that the biomarker panel could discriminate between menu plans by detecting distinctive changes in the concentration in urine of targeted metabolites. Quantitative relationships between four biomarker concentrations in urine and dietary intake are shown. Conclusion Design concepts for an analytical strategy are demonstrated, allowing simultaneous quantification of a comprehensive panel of chemically‐diverse biomarkers of a wide range of commonly‐consumed foods. It is proposed that integration of self‐reported dietary recording tools with biomarker approaches will provide more robust assessment of dietary exposure.