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Chlorogenic Acid Suppresses miR‐155 and Ameliorates Ulcerative Colitis through the NF‐κB/NLRP3 Inflammasome Pathway
Author(s) -
Zeng Junhao,
Zhang Dengqing,
Wan Xiaoyu,
Bai Yuanling,
Yuan Chengfu,
Wang Ting,
Yuan Ding,
Zhang Changcheng,
Liu Chaoqi
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202000452
Subject(s) - inflammasome , chemistry , downregulation and upregulation , caspase 1 , colitis , nf κb , signal transduction , inflammation , receptor , cancer research , immunology , biology , biochemistry , gene
Scope The over‐activation of the nucleotide‐binding domain like receptor protein 3 (NLRP3) inflammasome plays an important role in the pathogenesis of ulcerative colitis (UC). Chlorogenic acid (CGA) exposure is identified as an effective strategy for repressing inflammatory responses. Methods and results In this study, the NLRP3 inflammasome model with LPS/ATP‐induced RAW264.7 cells in vitro and dextran‐sulfate‐sodium (DSS)‐induced colitis in mice are used to evaluate the effect of CGA on NLRP3 inflammasome‐related signaling. The results suggest that CGA suppressed the expression of NLRP3 inflammasome‐related genes (apoptosis‐associated speck‐like protein containing CARD (ASC), cysteine‐requiring aspartate protease (Caspase)‐1 p45, Caspase‐1 p20, pro‐/cleaved‐interleukin (IL)‐1β, pro‐/cleaved‐IL‐18), p‐nuclear factor kappa B (NF‐κB) protein, and miR‐155 in mice with colitis. Gain‐ and loss‐of‐function studies of miR‐155 are performed to elucidate its role in inflammation. Moreover, activation of the NF‐κB/NLRP3 inflammasome pathway and miR‐155 expression is investigated. CGA exposure in lipopolysaccharide (LPS)/adenosine triphosphate (ATP)‐stimulated RAW264.7 cells leads to a decrease in p‐NK‐κB and NLRP3 inflammasome‐related proteins, which is dependent on the downregulation of miR‐155 expression. Conclusions These findings indicate that CGA prevented colitis by downregulating miR‐155 expression and inactivating the NF‐κB/NLRP3 inflammasome pathway in macrophages. The current study has promising therapeutic implications in the treatment of UC.