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Bilberry Supplementation after Myocardial Infarction Decreases Microvesicles in Blood and Affects Endothelial Vesiculation
Author(s) -
BrylGórecka Paulina,
Sathanoori Ramasri,
Arevström Lilith,
Landberg Rikard,
Bergh Cecilia,
Evander Mikael,
Olde Björn,
Laurell Thomas,
Fröbert Ole,
Erlinge David
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.202000108
Subject(s) - microvesicles , flow cytometry , microbiology and biotechnology , calcein , extracellular vesicle , microvesicle , western blot , in vitro , myocardial infarction , endothelium , chemistry , medicine , immunology , biology , biochemistry , microrna , gene , membrane
Scope Diet rich in bilberries is considered cardioprotective, but the mechanisms of action are poorly understood. Cardiovascular disease is characterized by increased proatherogenic status and high levels of circulating microvesicles (MVs). In an open‐label study patients with myocardial infarction receive an 8 week dietary supplementation with bilberry extract (BE). The effect of BE on patient MV levels and its influence on endothelial vesiculation in vitro is investigated. Methods and results MVs are captured with acoustic trapping and platelet‐derived MVs (PMVs), as well as endothelial‐derived MVs (EMVs) are quantified with flow cytometry. The in vitro effect of BE on endothelial extracellular vesicle (EV) release is examined using endothelial cells and calcein staining. The mechanisms of BE influence on vesiculation pathways are studied by Western blot and qRT‐PCR. Supplementation with BE decreased both PMVs and EMVs. Furthermore, BE reduced endothelial EV release, Akt phosphorylation, and vesiculation‐related gene transcription. It also protects the cells from P2X 7 ‐induced EV release and increase in vesiculation‐related gene expression. Conclusion BE supplementation improves the MV profile in patient blood and reduces endothelial vesiculation through several molecular mechanisms related to the P2X 7 receptor. The findings provide new insight into the cardioprotective effects of bilberries.