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The Effect of Lactobacillus fermentum ME‐3 Treatment on Glycation and Diabetes Complications
Author(s) -
Guilbaud Axel,
Howsam Michael,
NiquetLéridon Céline,
Delguste Florian,
Fremont Marc,
Lestavel Sophie,
Maboudou Patrice,
Garat Anne,
Schraen Susanna,
Onraed Brigitte,
Foligné Benoît,
Boulanger Éric,
Tessier Frédéric J
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201901018
Subject(s) - glycation , diabetes mellitus , lactobacillus fermentum , medicine , chemistry , endocrinology , type 2 diabetes , lactobacillus casei , probiotic , biochemistry , lactic acid , biology , bacteria , fermentation , genetics , lactobacillus plantarum
Scope Type 2 diabetes (T2D) induces organ damage associated with glycation, among other metabolic pathways. While therapeutic strategies have been tested to reduce the formation and impact of glycation products, results remain equivocal. Anti‐diabetic therapies using probiotics have been proposed, but their effect upon glycation has not been reported. Here, the effects of the bacterial strain Lactobacillus fermentum ME‐3 on glycation and T2D‐related complications in a mouse model of T2D are investigated. Methods & Results Wild‐type LepR db/+ and diabetic LepR db/db littermates receive a daily gavage of either water or the probiotic ME‐3 strain (10 10 CFU). Glycation markers, fructoselysine‐derived furosine (FL‐furosine) and carboxymethyllysine (CML), are quantified in four major organs and plasma using stable‐isotope dilution LC–MS/MS. After 12 weeks of ME‐3 treatment, diabetic mice gain less weight and exhibit an apparently improved glucose tolerance. The ME‐3 treatment reduces median renal levels of FL‐furosine in both genotypes by 12–15%, and renal and pulmonary free‐CML in diabetic mice by 30% and 18%, respectively. Attenuated hepatic steatosis and an improved plasma lipid profile are also observed with treatment in both genotypes, while the gut microbiota profile is unchanged. Conclusion L. fermentum ME‐3 has therapeutic potential for reducing the formation/accumulation of some glycation products in kidneys and attenuating some common diabetes‐related complications.