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Procyanidin C1 Inhibits Melanoma Cell Growth by Activating 67‐kDa Laminin Receptor Signaling
Author(s) -
Bae Jaehoon,
Kumazoe Motofumi,
Murata Kyosuke,
Fujimura Yoshinori,
Tachibana Hirofumi
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201900986
Subject(s) - protein phosphatase 2 , dephosphorylation , phosphatase , microbiology and biotechnology , cell growth , biology , kinase , stress fiber , protein kinase a , receptor , phosphorylation , biochemistry , cell , cytoskeleton
Scope Procyanidin C1 (PC1) is an epicatechin trimer found mainly in grapes that is reported to provide several health benefits. However, little is known about the molecular mechanisms underlying these benefits. The aim of this study is to demonstrate the molecular mechanisms by which PC1 operates. Methods and results A 67‐kDa laminin receptor (67LR) is identified as a cell surface receptor of PC1, with a Kd value of 2.8 µ m . PC1 induces an inhibitory effect on growth, accompanied by dephosphorylation of the C‐kinase potentiated protein phosphatase‐1 inhibitor protein of 17 kDa (CPI17) and myosin regulatory light chain (MRLC) proteins, followed by actin cytoskeleton remodeling in melanoma cells. These actions are mediated by protein kinase A (PKA) and protein phosphatase 2A (PP2A) activation once PC1 is bound to 67LR. Conclusion It is demonstrated that PC1 elicits melanoma cell growth inhibition by activating the 67LR/PKA/PP2A/CPI17/MRLC pathway.